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Acrylamide 98%(Microbiological Grade)

  • Acrylamide 98%(Microbiological Grade)

Acrylamide 98%(Microbiological Grade)

Acrylamide 98% (Microbiological Grade): High assay, low heavy metals, excellent solubility. Junteng Chem supplies consistent grade for lab use.
Tel: +86 18596277178
Tel: +86 15588955913
Tel: +86 17663494691
WhatsApp: +86 18806419898
Email: victoria@juntengchem.com

Product Parameters

ITEMINDEX
AppearanceWhite crystal powder (flake)
Content (%)≥98
Moisture(%)≤1
Fe(PPM)≤1
Cu(PPM)0
Color 20%Solution(Hazen)≤20
Water Insoluble(%)≤0.005%
Inhibitor(PPM)≤10
Conductivity of 40%solution(μs/cm)≤20
PH6-8


Basic Properties

  • Solubility: Easily soluble in water (approx. 215 g/100 mL, 30°C), ethanol, methanol, and acetone; slightly soluble in ethyl acetate and chloroform; insoluble in benzene and heptane.


  • pH (1% aqueous solution): Approx. 5.5-7.0


  • Stability: Stable in the solid state, but acrylamide in aqueous solution will slowly hydrolyze (to produce acrylic acid and ammonia). Polymerization is likely to occur upon exposure to light, heat, or prolonged storage.


  • Polymerization characteristics: In the presence of initiators (such as ammonium persulfate) and accelerators (such as TEMED), it can form polyacrylamide gels, used for protein and nucleic acid electrophoresis.


  • Toxicity: Neurotoxin, can be absorbed through the skin; irritating to eyes, skin, and respiratory tract; suspected carcinogen (IARC Class 2A).


  • Storage conditions: Store in a cool, dry place, protected from light (generally recommended at 2-8°C or room temperature; see product instructions for details).


Main Applications

1. Polyacrylamide Gel Electrophoresis in Microbiology


Core Application: Analysis of microbial proteins (e.g., bacterial lysates, recombinant proteins).


Why Choose Microbial Grade: Ordinary grade acrylamide may contain trace impurities that inhibit PCR or culture. Microbial grade ensures that no factors inhibiting downstream microbial detection or culture are introduced during sample processing before electrophoresis.


Typical Concentration: 10-15% separating gel is prepared for separating small molecule proteins (5-100 kDa).


2. Bacterial Capsule and Polysaccharide Analysis


Specialized Use: Preparation of high-resolution polyacrylamide gels for analysis of extracellular polysaccharides or lipopolysaccharides (LPS) secreted by microorganisms.


Technical Advantages: 98% purity combined with microbial grade quality control avoids interference from metal ions in the silver staining method for polysaccharide band development.


3. Preparation of Carriers for Immobilized Microbial Cells


Innovative Application: By polymerizing acrylamide monomers with cross-linking agents to form a three-dimensional hydrogel network, it is used to immobilize bacteria or yeast for:


Biodegradation in wastewater treatment.


Fermentation for producing metabolites (such as lactic acid and ethanol).


The critical aspect of microbial grade: Residual monomers are toxic to microorganisms. 98% purity is validated, ensuring that residual toxicity after polymerization is below the microbial inhibition threshold.


4. DNA/RNA extraction pretreatment in environmental microbiology


Specific process: Using low-concentration acrylamide (e.g., 0.5-2%) for co-precipitation to purify microbial nucleic acids from environmental samples (soil, sludge) with high humic acid content.


5. Gel migration assay in microbial resistance research


Application scenario: Studying the binding of transcription factors or regulatory proteins to the promoter DNA of antibiotic resistance genes.


Specifications: Microbial grade avoids the introduction of unknown enzymes or chemical impurities that interfere with protein-DNA interactions.


Safety Precautions

Company Profile

Junteng Chemical is a professional chemical raw materials trading company dedicated to providing customers with one-stop procurement services. Through strict supplier, supply chain, and after-sales management, the company ensures that product quality, transportation, and use meet customer needs. Furthermore, the company's technical team offers customized solutions and support services free of charge.

 

The company has projects in the pharmaceutical, pesticide, petrochemical, construction, food additives, wastewater treatment, adhesives, inks, paints, coatings, plastic and rubber processing, detergent raw materials, fertilizer and textile mills, and more. Junteng Chemical sources products of stable and reliable quality, collaborating with domestic and international companies such as Luxi Group, Weiyuan Chemical, Qilu Petrochemical, Dongying Lihuayi, Yanshan Petrochemical, Sinopec, Shijiazhuang Refinery, Qingdao Petrochemical, Sailboat, Dow Chemical, BASF, South Korea, Taiwan, and Saudi Arabia. Our stable supply, superior product quality, and mature logistics have earned us a wide clientele. We look forward to collaborating with all of you for a mutually beneficial future.

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Company advantages

1

One-Stop Procurement
Platform

We integrate resources across all productcategories, including organic solvents,resins and rubbers, and inorganicchemicals,and strictly control suppliers.supply chains, and after-sales processes toensure stable quality, efficienttransportation,and worry-free use,simplifying your procurement process.

2

Professional Team TechnicalEmpowerment

Equipped with an experienced technicalteam, we provide free personalizedsolutions and professional technicalsupport to customers in varlous industrie,such as pharmaceuticals, petrochemicals,coatings and inks, and sewage treatment,.helping you solve problems and improveefficiency.

3

Global supply ChainSecurity

We work with top domestic and foreignchemical giants such as Luxi Group, QiluPetrochemical, Sinopec, BASF of Germany,and Dow of the United States to establisha stable and reliable high-quality supplynetwork and a mature logistics system toensure timely and safe delivery of
products.

4

Deep Win-Win CooperationConcept

Adhering to the concept of "integrity.immediate action, professionalism,innovation and win-win" and taking"customer success and altruism" as ourmission, we are committed to establishinglong-term strategic partnerships andcreating a better future through excellentproducts and services.

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